Glowin’ Hormones

I used an excerpt from this book to learn about chemiluminescent assays for cortisol. It had easy to follow steps and some great pictures. Basically, serum samples are treated with certain proteins and enzymes that bind to cortisol and that cause it to break away from plasma proteins. Then an electrode is inserted into the sample and a charge is run through it. The brighter the chemiluminescence, the less cortisol is in the same

 

Nussey, S. S., & Whitehead, S. A. (2001). Endocrinology: An Integrated Approach. United Kingdom: Bios Scientific Publ

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